How can you tell if a streak plate is contaminated?
If some of your agar plates become contaminated, you can often tell by examining the plate how contamination took place. If the contaminants are imbedded in the agar, the contaminant was probably poured with the medium.
How do bacteria spread on agar plates?
The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily.
What is a disadvantage of the streak plate technique?
Disadvantages. The streak plate method does not work with high volumes of organisms. It will not enable you to get a concentration count. It requires huge storage space and there is a possibility that your incubator cannot accommodate a large volume of petri plate.
Can bacteria grow on a streak plate?
After incubation, bacterial growth is visible as colonies in and on the agar of a pour plate. Can some bacteria grow on the streak plate and not be seen if the pour plate technique is used? Yes, the pour plate is O2 limited. Thus, some bacteria will only grow on the streak plate as it provides ample O2.
How do you streak a plate with bacteria?
- Hold loop with bacterial sample parallel to fresh plate.
- Gently rub loop across surface spreading bacteria thinly throughout.
- Flip loop on other side or even gently drag edges across plate if visible bacteria still needs to be delivered.
What happens if you incubate bacteria too long?
If a bacterial culture is left in the same media for too long, the cells use up the available nutrients, excrete toxic metabolites, and eventually the entire population will die. Thus bacterial cultures must be periodically transferred, or subcultured, to new media to keep the bacterial population growing.
Which is better pour plate or spread plate?
The main difference between pour plate and spread plate is that the molten agar is poured on to the inoculum during the preparation of the pour plate whereas inoculum is spread on the surface of the solidified agar during the preparation of the spread plate.
What is the difference between streak plate and spread plate technique?
The key difference between streak plate and spread plate is that the streak plate is used to isolate and purify a particular bacterial species from a mixture of bacteria while the spread plate is used to enumerate and quantify bacteria in a sample.
How long does it take for bacteria to grow on agar plate?
The ideal temperature for growing bacteria is between 70 and 98 degrees F (20-37 degrees C). If necessary, you can place the Petri dishes in a cooler location, but the bacteria will grow a lot more slowly. Leave the bacteria to develop for 4-6 days, as this will give the cultures enough time to grow.
What is the primary purpose of the pour plate?
Pour plates allow micro-organisms to grow both on the surface and within the medium. Most of the colonies grow within the medium and are small in size and may be confluent. The few colonies that grow on the surface are of the same size and appearance as those on a streak plate.
What is the purpose of doing an isolation streak plate?
The streak plate technique is the most popular method for isolating specific bacteria from a sample containing a mixture of microorganisms. The technique essentially dilutes the number of organisms and reduces their density. It allows microbiologists to distinguish and isolate individual bacterial colonies.
Why must you Flame the loop between streaks?
Flaming the loop between streaks ensures that the loop starts clean and that only this small amount of bacteria is used to inoculate the next quadrant.
What will a streak plate with two species of bacteria look like?
What will a streak plate with two species of bacteria look like? Bacteria will be in a straight line, the will be separate from one another. where will the colonies be located in a pour plate? Throughout the agar, the will be larger at the surface because they have more room to grow.
How long is a bacterial generation?
For most known bacteria that can be cultured, generation times range from about 15 minutes to 1 hour. Symbionts such as Rhizobium tend to have longer generation times. Many lithotrophs, such as the nitrifying bacteria, also have long generation times.
What would happen if you forgot to sterilize your loop in between each quadrant streak?
What is a bacterial colony? What would happen if you forgot to sterilize your loop in between each quadrant streak? You would spread a lot of bacteria back into quadrant one and probably not see isolated colonies.